PKMS Knowledge Center

Pharmacokinetics and Mass Spectrometry Core Facility (PKMS) is a shared core resource facility in UM, and serves the scientific research community at UM and beyond with state-of-the-art expertise, methodology, and instrumentation. We are pleased to offer a complete PK service to your research projects with different options including collaboration, Fee-for-Service (FFS) and self-service. Please check our complete PK analysis service - from in vitro and in vivo drug discovery to bioanalysis and Mass spectrometry imaging.

Pharmacokinetics (PK) is the study of the time course of drug’s absorption, distribution, metabolism, and excretion (ADME), what the body interact with a drug, related to the movement of drug into, pass, and out of the body. In order for a drug to be effective in the body, enough of the active form must reach the target to elicit the desired effect. The compound with the best activity against the target enzyme or receptor may have very poor ADME characteristics such as metabolic instability or poor intestinal absorption. When choosing a lead candidate from multiple compounds, the activity of the drug in the efficacy screen must be balanced with acceptable ADME and stability characteristics. Pharmacokinetic studies with mass spectrometry bioanalysis can provide the quantitative information about the half-life of the administrated compound from in vitro and in vivo test, and more useful information of how quickly it can be metabolized or excreted by enzymatically or nonenzymatically. To track the drug accumulation and distribution in organism from in vivo PK study, the mass spectrometry imaging and quantification can come up with innovative solutions to quantify dynamic drug exposures in all targeted tissues, or using artificial intelligence modeling to predict the drug exposure in disease or normal organs.   

Instruments application notes from different company to describe the capability of the each instrument. (UM PKMS core own those cutting-edge technology and instrument to perform similar studies.) 

  1. SCIEX Triple Quad 5500 LC-MS/MS --- Highly Sensitive and Robust Quantification Method for Ethinyl Estradiol and Drospirenone in plasma    https://sciex.com/Documents/tech%20notes/Highly_Sensitive_and_Robust_Quantification.pdf
  2. SCIEX Triple Quad 5500 Qtrap system --- In Vivo Metabolic Profiling of Carbamazepine Using the QTRAP® 5500 System and LightSight® Software v2.2   https://sciex.com/Documents/Downloads/Literature/mass-spectrometry-Carbamazepine-profiling-1036610.pdf
  3. SCIEX X500R QTOF system --- Using MS/MSall with SWATH Acquisition For Forensic Designer Drug Analysis with SCIEX X500R QTOF system and SCIEX OS Software https://sciex.com/Documents/tech%20notes/forensic-designer-drug-analysis.pdf
  4. SCIEX X500R QTOF system --- Rapid Metabolite Identification using MetabolitePilot™ Software and TripleTOF™ 5600 system   https://sciex.com/Documents/Downloads/Literature/Rapid_Metabolite_Identification_using_MetabolitePilot_Software.pdf
  5. SYNAPT G2-Si HDMS QTOF system --- Targeted High Resolution Quantification with Tof-MRM and HD-MRM http://www.waters.com/webassets/cms/library/docs/720004728EN.pdf 
  6. Waters SYNAPT G2-Si HDMS QTOF system --- Qualitative and Quantitative Metabolite Identification for Verapamil in Rat Plasma Using a UPLC/SYNAPT G2 HDMS Strategy with MSE   http://www.waters.com/webassets/cms/library/docs/720003492en.pdf
  7.  Waters SYNAPT G2-Si HDMS QTOF system --- Ion Mobility Separation Coupled With Desorption Electrospray Ionization Mass Spectrometry for High Specificity MS Imaging   http://www.waters.com/webassets/cms/library/docs/720005525en.pdf
  8. Waters SYNAPT G2-Si HDMS QTOF system --- Lipid Visualization and Identification Through Collisional Cross Section Aided Correlation of MS Imaging and Ex Situ MS Data for MS-MS Identification   http://www.waters.com/webassets/cms/library/docs/720005354en.pdf

 

Mass spectrometry imaging (MSI) is an established technique that has revolutionized and untargeted biomedical and pharmacological investigations into the spatial distribution of molecular species in a variety of samples. 

We have a state of the art MALDI/DESI SYNAPT G2-Si High Definition Mass Spectrometry (Waters®) in our Pharmacokinetics and Mass Spectrometry Core Facility (PKMS). Using the MALDI and DESI ion source together with T-Wave ion mobility technology, our experienced team of mass spectrometry imagers can provide distribution profiling information for a wide range of sample types, such as tissue specimens, industrial and biological materials, horticultural specimens, and microbiological populations. We offer MSI sample preparation, including cryo-sectioning and microtome techniques, histological staining, and tissue microscopy. We can also combine imaging data with data acquired by different analytical techniques to deliver more comprehensive results. Our team works with you to identify MSI approaches to address your application requirements.

  • Drug development
  • Pharmacokinetics: Drug and Metabolite Distribution, 2D and 3D label free Imaging
  • Disease characterization and biomarker investigations
  • Carcinomas
  • Inter-and intra-tumor heterogeneity
  • Inflammatory diseases
  • Fibrosis
  • Bacterial infections and manifestations
  • Neurological diseases

Instruments application notes from Waters® to describe the capability of the SYNAPT G2-Si High Definition Mass Spectrometry.

  1. Biomarker Discovery Directly from Tissue Xenograph Using High Definition Imaging MALDI Combined with Multivariate Analysis. http://www.waters.com/webassets/cms/library/docs/720004873en.pdf
  2. Data Independent MALDI Imaging HDMSE for Visualization and Identification of Lipids Directly from a Single Tissue Section. http://www.waters.com/webassets/cms/library/docs/720004471en.pdf
  3. Distribution of Biomarkers of Interest in Rat Brain Tissues Using High Definition MALDI Imaging. http://www.waters.com/webassets/cms/library/docs/720004135en.pdf
  4. MALDI Imaging of Distribution of Xanthohumol and Its Metabolites in Rat Tissues. http://www.waters.com/webassets/cms/library/docs/720003707en.pdf
  5. Successful Application for Distribution Imaging of Chloroquine Ocular Tissue in Pigmented Rat Using MALDI-Imaging Quadrupole Time-of-Flight Mass Spectrometry. http://www.waters.com/waters/library.htm?locale=en_SE&cid=10010845&lid=10180348
  6. MALDI-Ion Mobility Separation-Mass Spectrometry Imaging of Glucose-Regulated Protein 78 kDa (Grp78) in Human Formalin-Fixed, Paraffin-Embedded Pancreatic Adenocarcinoma Tissue Sections. https://pubs.acs.org/doi/abs/10.1021/pr900522m
  7. Tissue Imaging of Pharmaceuticals by Ion Mobility Mass Spectrometry. http://www.waters.com/webassets/cms/library/docs/720003216en.pdf
  8. Direct Tissue Imaging and Characterization of Phospholipids Using MALDI SYNAPT HDMS System. http://www.waters.com/webassets/cms/library/docs/720002444en.pdf
  9. Localizing Diazepam and its Metabolite in Rat Brain Tissue by Imaging Mass Spectrometry using MALDI Q-Tof Premier MS. http://www.waters.com/webassets/cms/library/docs/720002447en.pdf
  10. Matrix-Assisted Laser Desorption/Ionization-Ion Mobility Separation-Mass Spectrometry Imaging of Vinblastine in Whole Body Tissue Sections. https://pubs.acs.org/doi/abs/10.1021/ac8015467
Pharmacokinetics

Pharmacokinetics (PK) is the study of the time course of drug’s absorption, distribution, metabolism, and excretion (ADME), what the body interact with a drug, related to the movement of drug into, pass, and out of the body. In order for a drug to be effective in the body, enough of the active form must reach the target to elicit the desired effect. The compound with the best activity against the target enzyme or receptor may have very poor ADME characteristics such as metabolic instability or poor intestinal absorption. When choosing a lead candidate from multiple compounds, the activity of the drug in the efficacy screen must be balanced with acceptable ADME and stability characteristics. Pharmacokinetic studies with mass spectrometry bioanalysis can provide the quantitative information about the half-life of the administrated compound from in vitro and in vivo test, and more useful information of how quickly it can be metabolized or excreted by enzymatically or nonenzymatically. To track the drug accumulation and distribution in organism from in vivo PK study, the mass spectrometry imaging and quantification can come up with innovative solutions to quantify dynamic drug exposures in all targeted tissues, or using artificial intelligence modeling to predict the drug exposure in disease or normal organs.   

Instruments application notes from different company to describe the capability of the each instrument. (UM PKMS core own those cutting-edge technology and instrument to perform similar studies.) 

  1. SCIEX Triple Quad 5500 LC-MS/MS --- Highly Sensitive and Robust Quantification Method for Ethinyl Estradiol and Drospirenone in plasma    https://sciex.com/Documents/tech%20notes/Highly_Sensitive_and_Robust_Quantification.pdf
  2. SCIEX Triple Quad 5500 Qtrap system --- In Vivo Metabolic Profiling of Carbamazepine Using the QTRAP® 5500 System and LightSight® Software v2.2   https://sciex.com/Documents/Downloads/Literature/mass-spectrometry-Carbamazepine-profiling-1036610.pdf
  3. SCIEX X500R QTOF system --- Using MS/MSall with SWATH Acquisition For Forensic Designer Drug Analysis with SCIEX X500R QTOF system and SCIEX OS Software https://sciex.com/Documents/tech%20notes/forensic-designer-drug-analysis.pdf
  4. SCIEX X500R QTOF system --- Rapid Metabolite Identification using MetabolitePilot™ Software and TripleTOF™ 5600 system   https://sciex.com/Documents/Downloads/Literature/Rapid_Metabolite_Identification_using_MetabolitePilot_Software.pdf
  5. SYNAPT G2-Si HDMS QTOF system --- Targeted High Resolution Quantification with Tof-MRM and HD-MRM http://www.waters.com/webassets/cms/library/docs/720004728EN.pdf 
  6. Waters SYNAPT G2-Si HDMS QTOF system --- Qualitative and Quantitative Metabolite Identification for Verapamil in Rat Plasma Using a UPLC/SYNAPT G2 HDMS Strategy with MSE   http://www.waters.com/webassets/cms/library/docs/720003492en.pdf
  7.  Waters SYNAPT G2-Si HDMS QTOF system --- Ion Mobility Separation Coupled With Desorption Electrospray Ionization Mass Spectrometry for High Specificity MS Imaging   http://www.waters.com/webassets/cms/library/docs/720005525en.pdf
  8. Waters SYNAPT G2-Si HDMS QTOF system --- Lipid Visualization and Identification Through Collisional Cross Section Aided Correlation of MS Imaging and Ex Situ MS Data for MS-MS Identification   http://www.waters.com/webassets/cms/library/docs/720005354en.pdf

 

PKMS.jpg

Mass spectrometry imaging

Mass spectrometry imaging (MSI) is an established technique that has revolutionized and untargeted biomedical and pharmacological investigations into the spatial distribution of molecular species in a variety of samples. 

We have a state of the art MALDI/DESI SYNAPT G2-Si High Definition Mass Spectrometry (Waters®) in our Pharmacokinetics and Mass Spectrometry Core Facility (PKMS). Using the MALDI and DESI ion source together with T-Wave ion mobility technology, our experienced team of mass spectrometry imagers can provide distribution profiling information for a wide range of sample types, such as tissue specimens, industrial and biological materials, horticultural specimens, and microbiological populations. We offer MSI sample preparation, including cryo-sectioning and microtome techniques, histological staining, and tissue microscopy. We can also combine imaging data with data acquired by different analytical techniques to deliver more comprehensive results. Our team works with you to identify MSI approaches to address your application requirements.

  • Drug development
  • Pharmacokinetics: Drug and Metabolite Distribution, 2D and 3D label free Imaging
  • Disease characterization and biomarker investigations
  • Carcinomas
  • Inter-and intra-tumor heterogeneity
  • Inflammatory diseases
  • Fibrosis
  • Bacterial infections and manifestations
  • Neurological diseases

Instruments application notes from Waters® to describe the capability of the SYNAPT G2-Si High Definition Mass Spectrometry.

  1. Biomarker Discovery Directly from Tissue Xenograph Using High Definition Imaging MALDI Combined with Multivariate Analysis. http://www.waters.com/webassets/cms/library/docs/720004873en.pdf
  2. Data Independent MALDI Imaging HDMSE for Visualization and Identification of Lipids Directly from a Single Tissue Section. http://www.waters.com/webassets/cms/library/docs/720004471en.pdf
  3. Distribution of Biomarkers of Interest in Rat Brain Tissues Using High Definition MALDI Imaging. http://www.waters.com/webassets/cms/library/docs/720004135en.pdf
  4. MALDI Imaging of Distribution of Xanthohumol and Its Metabolites in Rat Tissues. http://www.waters.com/webassets/cms/library/docs/720003707en.pdf
  5. Successful Application for Distribution Imaging of Chloroquine Ocular Tissue in Pigmented Rat Using MALDI-Imaging Quadrupole Time-of-Flight Mass Spectrometry. http://www.waters.com/waters/library.htm?locale=en_SE&cid=10010845&lid=10180348
  6. MALDI-Ion Mobility Separation-Mass Spectrometry Imaging of Glucose-Regulated Protein 78 kDa (Grp78) in Human Formalin-Fixed, Paraffin-Embedded Pancreatic Adenocarcinoma Tissue Sections. https://pubs.acs.org/doi/abs/10.1021/pr900522m
  7. Tissue Imaging of Pharmaceuticals by Ion Mobility Mass Spectrometry. http://www.waters.com/webassets/cms/library/docs/720003216en.pdf
  8. Direct Tissue Imaging and Characterization of Phospholipids Using MALDI SYNAPT HDMS System. http://www.waters.com/webassets/cms/library/docs/720002444en.pdf
  9. Localizing Diazepam and its Metabolite in Rat Brain Tissue by Imaging Mass Spectrometry using MALDI Q-Tof Premier MS. http://www.waters.com/webassets/cms/library/docs/720002447en.pdf
  10. Matrix-Assisted Laser Desorption/Ionization-Ion Mobility Separation-Mass Spectrometry Imaging of Vinblastine in Whole Body Tissue Sections. https://pubs.acs.org/doi/abs/10.1021/ac8015467

Listing Row

Tuesday, May 10, 2016
Tuesday, May 10, 2016