NMR Metabolomics Laboratory
- Sample Submission
The NMR Core routinely runs quantitative untargeted metabolomics on whole blood, serum, plasma, urine, cell media. Please feel free to contact Larisa Yeomans firstname.lastname@example.org or Kathleen Stringer email@example.com with questions about your samples and study planning, and when possible, prior to your sample collection. NMR metabolomics is a powerful platform but not very sensitive, hence ideally we need a minimum volume of 600uL of biofluid and when possible one or two technical replicates.
- Sample process
After a conversation on the feasibility of running your samples by NMR, we will send you a quote, after you accept it, we can receive your samples (please bring them in dry ice), and update their information in our freezers using the unviersity freezer inventory 'Freezerworks'. Once your samples get scheduled to be processed they will be precipitated/extracted/lyophilized/filtered according with the SOP used for your biofluid and an internal standard or a known concentration (DSS-d6 or Formate) will be added. Metabolomics samples are run on the 500MHz NMR instrument in our Core.
After spectral acquisition, the NMR spectra is processed and profiled using commercially available Chenomx software which contains a library of hundreds of metabolites and each detectable metabolite is profiled to yield a metabolite concentration. At the end you will have a list of metabolites (30-50 metabolites depending on the biofluid) and their concentration in uM units.
University of Michigan investigators: $135 per sample
External investigators: $160 per sample plus the % IDC