Biochemical Nuclear Magnetic Resonance (BNMR) Services
Want to Know More About Your Small Molecules and Metabolomics?
We provide full-service consultation, sample processing and results. Our BNMR core is open to U-M faculty, graduate students, postdocs and external investigators. Get to know more about our BNMR capabilities. We are excited to work with you!
Small Molecule NMR Services
If you have questions about structure elucidation, what experiments you should run, how to plan your study or data processing, we can help you! We run 1D experiments: 1H and 13C, as well as one of the most useful 2D experiments, HSQC, and we process the data using Mestranova to explore new drug candidates.
Training
We encourage a hands-on experience so that you can run your experiments and access your data. To do that, we require a two-hour training session.
Sample Service
We offer sample services, prepping your sample(s) for you. This is the recommended way to ensure standards related to NMR tubes and deuterated solvents are met.
Sample Preparation
You can choose to prepare your samples. If you do, be sure you have a quality NMR tube and that your deuterated solvents from an open bottle are not too old.
Results
After running your samples, we will process your data using Mestranova and give you access to the raw data via email or Dropbox.
Do It Yourself
400 MHz
Internal U-M – $16 per hour (billed in 15 minutes increments)
External Users – $60 per hour
500 MHz
Internal U-M – $22 per hour (billed in 15 minutes increments)
External Users – $70 per hour
Consulting Rate
Internal- $80 per hour
External- $100 per hour
Spectroscopist
Internal U-M: $39 (sample service fee per sample) in addition to hourly fee for machine usage
External: $50 (sample service fee per sample) in addition to hourly fee for machine usage
- The instruments can be reserved by scheduling equipment time after you have logged into micores for the NMR lab.
- Once you are trained, you will be granted access to reserve instrument time for the 400MHz and the 500MHz.
- The 400MHz can be reserved for up to an hour from 8:00 am-7:00 pm during the week, and after that, you can reserve for overnight runs. You can reserve over the weekend for as long as you need to.
- The 500MHz can be reserved freely.
*College of Pharmacy graduate students and faculty have reservation priority
Metabolomic NMR Services
Need to run quantitative untargeted metabolomics? We routinely run samples on a broad range of biological fluids such as whole blood, serum, plasma urine and cell media.
Sample Collection
Please contact us to discuss your study plan before collecting and submitting samples. We will guide you on the volume and quantity of biofluid samples and study feasibility.
Sample Process
Send us your samples on dry ice, and we will store them until they are processed. We process samples following standard precipitation/ extraction/ lyophilization/ filtration procedures and add Formate using the 500MHz NMR instrument.
Results
After processing, samples are profiled using commercially available Chenomx software. At the end, you will have a list of metabolites (30-50) and their concentration in uM units.
Fees
U-M Investigator: $200
External User: Please contact Larissa Yeomans
